Patients with suspected acute coronary syndrome (ACS) require urgent evaluation. It is essential to establish whether the symptoms are a manifestation of an ACS and, if so, how likely it is for an adverse clinical event to occur.[2]Rao SV, O'Donoghue ML, Ruel M, et al. 2025 ACC/AHA/ACEP/NAEMSP/SCAI guideline for the management of patients with acute coronary syndromes: a report of the American College of Cardiology/American Heart Association Joint Committee on Clinical Practice Guidelines. Circulation. 2025 Apr;151(13):e771-862.
https://www.ahajournals.org/doi/full/10.1161/CIR.0000000000001309?rfr_dat=cr_pub++0pubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org
http://www.ncbi.nlm.nih.gov/pubmed/40014670?tool=bestpractice.com
Physicians need to first establish the patient's risk and follow current guidelines according to the initial risk assessment to choose an appropriate management strategy. The initial risk assessment includes the history, examination, ECG, and cardiac biomarkers.[2]Rao SV, O'Donoghue ML, Ruel M, et al. 2025 ACC/AHA/ACEP/NAEMSP/SCAI guideline for the management of patients with acute coronary syndromes: a report of the American College of Cardiology/American Heart Association Joint Committee on Clinical Practice Guidelines. Circulation. 2025 Apr;151(13):e771-862.
https://www.ahajournals.org/doi/full/10.1161/CIR.0000000000001309?rfr_dat=cr_pub++0pubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org
http://www.ncbi.nlm.nih.gov/pubmed/40014670?tool=bestpractice.com
[5]Byrne RA, Rossello X, Coughlan JJ, et al. 2023 ESC guidelines for the management of acute coronary syndromes. Eur Heart J. 2023 Oct 12;44(38):3720-826.
https://academic.oup.com/eurheartj/article/44/38/3720/7243210
[79]National Institute for Health and Care Excellence. Recent-onset chest pain of suspected cardiac origin: assessment and diagnosis. November 2016 [internet publication].
https://www.nice.org.uk/guidance/CG95
ECG
ECG is indicated as the first-line investigation in all patients. A 12-lead ECG should be performed and interpreted within 10 minutes of the point of first medical contact in any patient with suspected non-ST-elevation myocardial infarction (NSTEMI).[5]Byrne RA, Rossello X, Coughlan JJ, et al. 2023 ESC guidelines for the management of acute coronary syndromes. Eur Heart J. 2023 Oct 12;44(38):3720-826.
https://academic.oup.com/eurheartj/article/44/38/3720/7243210
[80]Writing Committee, Kontos MC, de Lemos JA, et al. 2022 ACC Expert Consensus Decision Pathway on the evaluation and disposition of acute chest pain in the emergency department: A report of the American College of Cardiology Solution Set Oversight Committee. J Am Coll Cardiol. 2022 Nov 15;80(20):1925-60.
https://www.sciencedirect.com/science/article/pii/S0735109722066189?via%3Dihub
http://www.ncbi.nlm.nih.gov/pubmed/36241466?tool=bestpractice.com
It is critical to immediate management to exclude ST-elevation myocardial infarction (STEMI). NSTEMI is indistinguishable from other types of ACS (STEMI or unstable angina) until ECG and biomarkers become available, because their pathophysiology and presentation are similar.[2]Rao SV, O'Donoghue ML, Ruel M, et al. 2025 ACC/AHA/ACEP/NAEMSP/SCAI guideline for the management of patients with acute coronary syndromes: a report of the American College of Cardiology/American Heart Association Joint Committee on Clinical Practice Guidelines. Circulation. 2025 Apr;151(13):e771-862.
https://www.ahajournals.org/doi/full/10.1161/CIR.0000000000001309?rfr_dat=cr_pub++0pubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org
http://www.ncbi.nlm.nih.gov/pubmed/40014670?tool=bestpractice.com
Typical ECG findings may be present, but many patients have a normal ECG at presentation and therefore serial ECGs, initially at 15- to 30-minute intervals, should be performed to detect the potential for development of ST-segment elevation or depression.[5]Byrne RA, Rossello X, Coughlan JJ, et al. 2023 ESC guidelines for the management of acute coronary syndromes. Eur Heart J. 2023 Oct 12;44(38):3720-826.
https://academic.oup.com/eurheartj/article/44/38/3720/7243210
Where present, ECG findings in NSTEMI may be highly variable. Typically >1 mm of ST depression is present in 2 or more contiguous leads. Other potential findings include dramatic new T-wave inversions (Wellens waves) or transient ST elevation. ECG changes of ST elevation or new left bundle branch block should be evaluated as STEMI.[Figure caption and citation for the preceding image starts]: ECG showing ST depressionFrom the personal collection of Dr Syed W. Yusuf and Dr Iyad N. Daher, Department of Cardiology, University of Texas, Houston; used with permission [Citation ends].
[Figure caption and citation for the preceding image starts]: ECG showing ST depressionFrom the personal collection of Dr Syed W. Yusuf and Dr Iyad N. Daher, Department of Cardiology, University of Texas, Houston; used with permission [Citation ends].
Continuous 12-lead ECG monitoring is a reasonable alternative to serial 12-lead recordings in patients whose initial ECG is nondiagnostic.[2]Rao SV, O'Donoghue ML, Ruel M, et al. 2025 ACC/AHA/ACEP/NAEMSP/SCAI guideline for the management of patients with acute coronary syndromes: a report of the American College of Cardiology/American Heart Association Joint Committee on Clinical Practice Guidelines. Circulation. 2025 Apr;151(13):e771-862.
https://www.ahajournals.org/doi/full/10.1161/CIR.0000000000001309?rfr_dat=cr_pub++0pubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org
http://www.ncbi.nlm.nih.gov/pubmed/40014670?tool=bestpractice.com
Supplemental ECG leads V7 through V9 may be useful in patients with nondiagnostic initial ECGs to rule out myocardial infarction (MI) due to left circumflex occlusion, and V3R and V4R may be useful to detect right ventricular MI.[5]Byrne RA, Rossello X, Coughlan JJ, et al. 2023 ESC guidelines for the management of acute coronary syndromes. Eur Heart J. 2023 Oct 12;44(38):3720-826.
https://academic.oup.com/eurheartj/article/44/38/3720/7243210
Less commonly, the ECG may reveal a tachyarrhythmia or bradyarrhythmia precipitated by the MI.
History and examination
Patients presenting with chest pain or discomfort need immediate assessment for current or past history of coronary artery disease (CAD) and traditional risk factors (e.g., age, sex, diabetes, hypertension, cocaine use) to triage them as high priority.[7]Writing Committee Members; Gulati M, Levy PD, Mukherjee D, et al. 2021 AHA/ACC/ASE/CHEST/SAEM/SCCT/SCMR guideline for the evaluation and diagnosis of chest pain: a report of the American College of Cardiology/American Heart Association Joint Committee On Clinical Practice Guidelines. J Am Coll Cardiol. 2021 Nov 30;78(22):e187-285.
https://www.sciencedirect.com/science/article/pii/S0735109721057958
http://www.ncbi.nlm.nih.gov/pubmed/34756653?tool=bestpractice.com
ACS is highly likely if there is a history of chest or left arm pain similar to previously documented angina pain, and a history of CAD (including MI). Angina pain is typically deep, poorly localized chest or arm pain described as a sensation of tightness, heaviness, aching, burning, pressure, or squeezing. The pain is most often retrosternal and can often radiate to the left arm, but may also radiate to the lower jaw, neck, both arms, back, and epigastrium, where it may mimic heartburn. Angina pain is associated with exertion or emotional stress (or less commonly with cold exposure) and relieved by rest.[7]Writing Committee Members; Gulati M, Levy PD, Mukherjee D, et al. 2021 AHA/ACC/ASE/CHEST/SAEM/SCCT/SCMR guideline for the evaluation and diagnosis of chest pain: a report of the American College of Cardiology/American Heart Association Joint Committee On Clinical Practice Guidelines. J Am Coll Cardiol. 2021 Nov 30;78(22):e187-285.
https://www.sciencedirect.com/science/article/pii/S0735109721057958
http://www.ncbi.nlm.nih.gov/pubmed/34756653?tool=bestpractice.com
Diaphoresis is a common associated symptom.[2]Rao SV, O'Donoghue ML, Ruel M, et al. 2025 ACC/AHA/ACEP/NAEMSP/SCAI guideline for the management of patients with acute coronary syndromes: a report of the American College of Cardiology/American Heart Association Joint Committee on Clinical Practice Guidelines. Circulation. 2025 Apr;151(13):e771-862.
https://www.ahajournals.org/doi/full/10.1161/CIR.0000000000001309?rfr_dat=cr_pub++0pubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org
http://www.ncbi.nlm.nih.gov/pubmed/40014670?tool=bestpractice.com
Shortness of breath is also common and is probably secondary to diminished cardiac output, but may also be due to underlying pulmonary congestion. Patients may express anxiety or appear anxious. They may also report a feeling of impending doom. Classically, events peak in the early morning hours, presumably due to hemodynamic stress caused by increased serum cortisol, adrenergic hormones, and platelet aggregation.[81]Manfredini R, Boari B, Salmi R, et al. Twenty-four-hour patterns in occurrence and pathophysiology of acute cardiovascular events and ischemic heart disease. Chronobiol Int. 2013 Mar;30(1-2):6-16.
https://www.tandfonline.com/doi/10.3109/07420528.2012.715843
http://www.ncbi.nlm.nih.gov/pubmed/23002808?tool=bestpractice.com
Patients may present with a range of noncharacteristic symptoms, any of which may be the sole presenting symptom. These include weakness, nausea, vomiting, abdominal pain, and syncope. These are more common in women, older people, and those with diabetes.[5]Byrne RA, Rossello X, Coughlan JJ, et al. 2023 ESC guidelines for the management of acute coronary syndromes. Eur Heart J. 2023 Oct 12;44(38):3720-826.
https://academic.oup.com/eurheartj/article/44/38/3720/7243210
Examination findings are usually nonspecific but may reveal hypertension or hypotension, the presence of third and fourth heart sounds, and paradoxical splitting of the second heart sound. Signs of heart failure (raised jugular venous pressure, bilateral crepitations on auscultation of the lungs) or cardiogenic shock may also be present, and these signify a worse prognosis.
Relief of chest pain with sublingual nitroglycerin is not necessarily diagnostic of myocardial ischemia and should not be used as a diagnostic criterion; other entities (e.g., esophageal spasm) may demonstrate a comparable response.[7]Writing Committee Members; Gulati M, Levy PD, Mukherjee D, et al. 2021 AHA/ACC/ASE/CHEST/SAEM/SCCT/SCMR guideline for the evaluation and diagnosis of chest pain: a report of the American College of Cardiology/American Heart Association Joint Committee On Clinical Practice Guidelines. J Am Coll Cardiol. 2021 Nov 30;78(22):e187-285.
https://www.sciencedirect.com/science/article/pii/S0735109721057958
http://www.ncbi.nlm.nih.gov/pubmed/34756653?tool=bestpractice.com
Pain described as sharp, fleeting (few seconds duration), or related to inspiration (pleuritic) or position, or which is shifting in location, suggests a lower likelihood of ischemia.[7]Writing Committee Members; Gulati M, Levy PD, Mukherjee D, et al. 2021 AHA/ACC/ASE/CHEST/SAEM/SCCT/SCMR guideline for the evaluation and diagnosis of chest pain: a report of the American College of Cardiology/American Heart Association Joint Committee On Clinical Practice Guidelines. J Am Coll Cardiol. 2021 Nov 30;78(22):e187-285.
https://www.sciencedirect.com/science/article/pii/S0735109721057958
http://www.ncbi.nlm.nih.gov/pubmed/34756653?tool=bestpractice.com
Initial tests (non-ECG)
In addition to the ECG, the following tests must also be performed in all patients.
Cardiac troponins: a rise in the levels of cardiac troponins (>99th percentile of normal) are diagnostic for the condition and the use of high-sensitivity cardiac troponin assays have shortened the assessment time interval.[80]Writing Committee, Kontos MC, de Lemos JA, et al. 2022 ACC Expert Consensus Decision Pathway on the evaluation and disposition of acute chest pain in the emergency department: A report of the American College of Cardiology Solution Set Oversight Committee. J Am Coll Cardiol. 2022 Nov 15;80(20):1925-60.
https://www.sciencedirect.com/science/article/pii/S0735109722066189?via%3Dihub
http://www.ncbi.nlm.nih.gov/pubmed/36241466?tool=bestpractice.com
This test is readily available at most institutions. Cardiac troponins are more sensitive and specific markers of cardiomyocyte damage than creatine kinase (CK), its myocardial band isoenzyme (CK-MB), and myoglobin. In patients with MI, troponin levels rise rapidly (usually within 1 hour from symptom onset if using high-sensitivity assays) and remain elevated for several days. Therefore, with the advent of high-sensitive troponin assays (hs-cTn), other biomarkers (e.g., CK, CK-MB, and myoglobin) should not be used to diagnose NSTEMI.[4]Thygesen K, Alpert JS, Jaffe AS, et al; Executive Group on behalf of the Joint European Society of Cardiology (ESC)/American College of Cardiology (ACC)/American Heart Association (AHA)/World Heart Federation (WHF) Task Force for the Universal Definition of Myocardial Infarction. Fourth universal definition of myocardial infarction (2018). J Am Coll Cardiol. 2018 Oct 30;72(18):2231-64.
http://www.onlinejacc.org/content/early/2018/08/27/j.jacc.2018.08.1038
http://www.ncbi.nlm.nih.gov/pubmed/30153967?tool=bestpractice.com
[5]Byrne RA, Rossello X, Coughlan JJ, et al. 2023 ESC guidelines for the management of acute coronary syndromes. Eur Heart J. 2023 Oct 12;44(38):3720-826.
https://academic.oup.com/eurheartj/article/44/38/3720/7243210
[7]Writing Committee Members; Gulati M, Levy PD, Mukherjee D, et al. 2021 AHA/ACC/ASE/CHEST/SAEM/SCCT/SCMR guideline for the evaluation and diagnosis of chest pain: a report of the American College of Cardiology/American Heart Association Joint Committee On Clinical Practice Guidelines. J Am Coll Cardiol. 2021 Nov 30;78(22):e187-285.
https://www.sciencedirect.com/science/article/pii/S0735109721057958
http://www.ncbi.nlm.nih.gov/pubmed/34756653?tool=bestpractice.com
[82]Society of Hospital Medicine - Adult Hospital Medicine. Eleven things physicians and patients should question. Choosing Wisely, an initiative of the ABIM Foundation. 2022 [internet publication].
https://web.archive.org/web/20230209055919/https://www.choosingwisely.org/societies/society-of-hospital-medicine-adult
[83]American Society for Clinical Pathology. Thirty five things physicians and patients should question. Choosing Wisely, an initiative of the ABIM Foundation. 2020 [internet publication].
https://web.archive.org/web/20230316185857/https://www.choosingwisely.org/societies/american-society-for-clinical-pathology
Similarly, for initial diagnostic purposes, routine measurement of additional biomarkers (e.g., heart-type fatty acid-binding protein [h-FABP] or copeptin) is not recommended in addition to hs-cTn.[5]Byrne RA, Rossello X, Coughlan JJ, et al. 2023 ESC guidelines for the management of acute coronary syndromes. Eur Heart J. 2023 Oct 12;44(38):3720-826.
https://academic.oup.com/eurheartj/article/44/38/3720/7243210
For interpretation of hs-cTn, European Society of Cardiology recommends using the 0/1 hour or 0/2 hour “rule in” and “rule out” algorithms, which classify patients into one of three pathways according to the results of their hs-cTn values at 0 hours (time of initial blood test) and 1 hour or 2 hours later.[5]Byrne RA, Rossello X, Coughlan JJ, et al. 2023 ESC guidelines for the management of acute coronary syndromes. Eur Heart J. 2023 Oct 12;44(38):3720-826.
https://academic.oup.com/eurheartj/article/44/38/3720/7243210
Rule-out pathway: for very low initial hs-cTn or no increase after 1/2 hours: these patients may be appropriate for early discharge and outpatient management.
Rule-in pathway: for high initial hs-cTN or an increase after 1-2 hours: most of these patients will require hospital admission and further evaluation.
Observe pathway: if neither of the above criteria is met: check hs-cTN at 3 hours and consider echocardiography.
Echocardiography: cardiac ultrasound may be useful for early triage of patients with suspected MI.[4]Thygesen K, Alpert JS, Jaffe AS, et al; Executive Group on behalf of the Joint European Society of Cardiology (ESC)/American College of Cardiology (ACC)/American Heart Association (AHA)/World Heart Federation (WHF) Task Force for the Universal Definition of Myocardial Infarction. Fourth universal definition of myocardial infarction (2018). J Am Coll Cardiol. 2018 Oct 30;72(18):2231-64.
http://www.onlinejacc.org/content/early/2018/08/27/j.jacc.2018.08.1038
http://www.ncbi.nlm.nih.gov/pubmed/30153967?tool=bestpractice.com
An urgent echocardiogram should always be performed in patients with cardiogenic shock or hemodynamic instability.[5]Byrne RA, Rossello X, Coughlan JJ, et al. 2023 ESC guidelines for the management of acute coronary syndromes. Eur Heart J. 2023 Oct 12;44(38):3720-826.
https://academic.oup.com/eurheartj/article/44/38/3720/7243210
[84]Edvardsen T, Asch FM, Davidson B, et al. Non-invasive imaging in coronary syndromes: recommendations of the European Association of Cardiovascular Imaging and the American Society of Echocardiography, in collaboration with the American Society of Nuclear Cardiology, Society of Cardiovascular Computed Tomography, and Society for Cardiovascular Magnetic Resonance. Eur Heart J Cardiovasc Imaging. 2022 Jan 24;23(2):e6-33.
https://academic.oup.com/ehjcimaging/article/23/2/e6/6423983
http://www.ncbi.nlm.nih.gov/pubmed/34751391?tool=bestpractice.com
Point-of-care transthoracic echocardiogram can also be used to look for regional wall motion abnormalities of the left ventricle in patients with an atypical presentation or equivocal ECG, look for mechanical complications of acute MI (such as left ventricular function, right ventricular function, ventricular septal rupture, left ventricular free wall rupture, acute mitral regurgitation, pericardial effusion, cardiac tamponade) and for evidence to suggest alternative etiologies associated with chest pain (e.g., acute aortic disease, pulmonary embolism).[2]Rao SV, O'Donoghue ML, Ruel M, et al. 2025 ACC/AHA/ACEP/NAEMSP/SCAI guideline for the management of patients with acute coronary syndromes: a report of the American College of Cardiology/American Heart Association Joint Committee on Clinical Practice Guidelines. Circulation. 2025 Apr;151(13):e771-862.
https://www.ahajournals.org/doi/full/10.1161/CIR.0000000000001309?rfr_dat=cr_pub++0pubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org
http://www.ncbi.nlm.nih.gov/pubmed/40014670?tool=bestpractice.com
[4]Thygesen K, Alpert JS, Jaffe AS, et al; Executive Group on behalf of the Joint European Society of Cardiology (ESC)/American College of Cardiology (ACC)/American Heart Association (AHA)/World Heart Federation (WHF) Task Force for the Universal Definition of Myocardial Infarction. Fourth universal definition of myocardial infarction (2018). J Am Coll Cardiol. 2018 Oct 30;72(18):2231-64.
http://www.onlinejacc.org/content/early/2018/08/27/j.jacc.2018.08.1038
http://www.ncbi.nlm.nih.gov/pubmed/30153967?tool=bestpractice.com
[5]Byrne RA, Rossello X, Coughlan JJ, et al. 2023 ESC guidelines for the management of acute coronary syndromes. Eur Heart J. 2023 Oct 12;44(38):3720-826.
https://academic.oup.com/eurheartj/article/44/38/3720/7243210
[84]Edvardsen T, Asch FM, Davidson B, et al. Non-invasive imaging in coronary syndromes: recommendations of the European Association of Cardiovascular Imaging and the American Society of Echocardiography, in collaboration with the American Society of Nuclear Cardiology, Society of Cardiovascular Computed Tomography, and Society for Cardiovascular Magnetic Resonance. Eur Heart J Cardiovasc Imaging. 2022 Jan 24;23(2):e6-33.
https://academic.oup.com/ehjcimaging/article/23/2/e6/6423983
http://www.ncbi.nlm.nih.gov/pubmed/34751391?tool=bestpractice.com
[85]Steeds RP, Garbi M, Cardim N, et al. EACVI appropriateness criteria for the use of transthoracic echocardiography in adults: a report of literature and current practice review. Eur Heart J Cardiovasc Imaging. 2017 Nov 1;18(11):1191-204.
https://academic.oup.com/ehjcimaging/article-abstract/18/11/1191/3052244
http://www.ncbi.nlm.nih.gov/pubmed/28329307?tool=bestpractice.com
A predischarge echocardiogram is indicated for all patients post-acute MI to assess left ventricular function after coronary reperfusion therapy and to guide prognostication.[86]National Institute for Health and Care Excellence. Acute coronary syndromes. Nov 2020 [internet publication].
https://www.nice.org.uk/guidance/ng185
[87]Loutfi M, Ashour S, El-Sharkawy E, et al. Identification of high-risk patients with non-ST segment elevation myocardial infarction using strain doppler echocardiography: correlation with cardiac magnetic resonance imaging. Clin Med Insights Cardiol. 2016 May 10;10:51-9.
https://pmc.ncbi.nlm.nih.gov/articles/PMC4863927
http://www.ncbi.nlm.nih.gov/pubmed/27199575?tool=bestpractice.com
Patients with a high index of suspicion who have negative serial ECGs and cardiac enzymes should be closely monitored in a telemetry or chest pain unit, as it may take time for cardiac markers to rise.[7]Writing Committee Members; Gulati M, Levy PD, Mukherjee D, et al. 2021 AHA/ACC/ASE/CHEST/SAEM/SCCT/SCMR guideline for the evaluation and diagnosis of chest pain: a report of the American College of Cardiology/American Heart Association Joint Committee On Clinical Practice Guidelines. J Am Coll Cardiol. 2021 Nov 30;78(22):e187-285.
https://www.sciencedirect.com/science/article/pii/S0735109721057958
http://www.ncbi.nlm.nih.gov/pubmed/34756653?tool=bestpractice.com
[88]Amsterdam EA, Wenger NK, Brindis RG, et al. 2014 AHA/ACC guideline for the management of patients with non-ST-elevation acute coronary syndromes: a report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines. J Am Coll Cardiol. 2014 Dec 23;64(24):e139-228.
https://www.jacc.org/doi/10.1016/j.jacc.2014.09.017
http://www.ncbi.nlm.nih.gov/pubmed/25260718?tool=bestpractice.com
Chest x-ray: indicated to exclude alternative causes for chest pain such as pneumothorax or a widened mediastinum in aortic dissection, or complications of ACS such as pulmonary edema due to heart failure.[80]Writing Committee, Kontos MC, de Lemos JA, et al. 2022 ACC Expert Consensus Decision Pathway on the evaluation and disposition of acute chest pain in the emergency department: A report of the American College of Cardiology Solution Set Oversight Committee. J Am Coll Cardiol. 2022 Nov 15;80(20):1925-60.
https://www.sciencedirect.com/science/article/pii/S0735109722066189?via%3Dihub
http://www.ncbi.nlm.nih.gov/pubmed/36241466?tool=bestpractice.com
CBC: hemoglobin and hematocrit measurements may help to evaluate a secondary cause of NSTEMI (e.g., acute blood loss, anemia) and to evaluate thrombocytopenia to estimate risk of bleeding.
BUN and serum creatinine: creatinine clearance should be estimated in NSTEMI patients and the doses of renally cleared drugs should be adjusted appropriately. In chronic kidney disease patients undergoing angiography, iso-osmolar contrast agents may be preferred.[89]Neumann FJ, Sousa-Uva M, Ahlsson A, et al; ESC Scientific Document Group. 2018 ESC/EACTS guidelines on myocardial revascularization. Eur Heart J. 2019 Jan 7;40(2):87-165.
https://academic.oup.com/eurheartj/advance-article/doi/10.1093/eurheartj/ehy394/5079120
http://www.ncbi.nlm.nih.gov/pubmed/30165437?tool=bestpractice.com
Serum electrolytes: electrolyte derangements may predispose to cardiac arrhythmias.
C-reactive protein (CRP): CRP is commonly ordered to rule out other causes of acute chest pain (e.g., pneumonia).
Liver function tests: useful if treatment with drugs that undergo hepatic metabolism is considered, and in the assessment of bleeding risk before starting anticoagulation. Impaired liver function tests may also suggest hepatic congestion in patients with concomitant heart failure.
Blood glucose levels: hyperglycemia is common in the setting of acute MI, with or without a history of diabetes.[90]Gholap NN, Mehta RL, Ng L, et al. Is admission blood glucose concentration a more powerful predictor of mortality after myocardial infarction than diabetes diagnosis? A retrospective cohort study. BMJ Open. 2012 Sep 25;2(5):e001596.
https://bmjopen.bmj.com/content/2/5/e001596.long
http://www.ncbi.nlm.nih.gov/pubmed/23015602?tool=bestpractice.com
There is controversy over whether tight glucose control may reduce risk of death and morbidity.[91]de Mulder M, Umans VA, Cornel JH, et al. Intensive glucose regulation in hyperglycemic acute coronary syndrome: results of the randomized BIOMarker study to identify the acute risk of a coronary syndrome-2 (BIOMArCS-2) glucose trial. JAMA Intern Med. 2013 Nov 11;173(20):1896-904.
https://jamanetwork.com/journals/jamainternalmedicine/fullarticle/1735896
http://www.ncbi.nlm.nih.gov/pubmed/24018647?tool=bestpractice.com
Risk stratification
ACS management requires continuous risk stratification for death or recurrent MI. The American College of Cardiology/American Heart Association recommend that patients with suspected ACS are risk stratified based on the likelihood of ACS and adverse outcome(s) to further triage and assist in the selection of treatment options, particularly in settings where high-sensitivity cardiac troponin assays are not available.[2]Rao SV, O'Donoghue ML, Ruel M, et al. 2025 ACC/AHA/ACEP/NAEMSP/SCAI guideline for the management of patients with acute coronary syndromes: a report of the American College of Cardiology/American Heart Association Joint Committee on Clinical Practice Guidelines. Circulation. 2025 Apr;151(13):e771-862.
https://www.ahajournals.org/doi/full/10.1161/CIR.0000000000001309?rfr_dat=cr_pub++0pubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org
http://www.ncbi.nlm.nih.gov/pubmed/40014670?tool=bestpractice.com
[7]Writing Committee Members; Gulati M, Levy PD, Mukherjee D, et al. 2021 AHA/ACC/ASE/CHEST/SAEM/SCCT/SCMR guideline for the evaluation and diagnosis of chest pain: a report of the American College of Cardiology/American Heart Association Joint Committee On Clinical Practice Guidelines. J Am Coll Cardiol. 2021 Nov 30;78(22):e187-285.
https://www.sciencedirect.com/science/article/pii/S0735109721057958
http://www.ncbi.nlm.nih.gov/pubmed/34756653?tool=bestpractice.com
Several risk scores exist that incorporate a number of variables such as clinical history, angina symptoms and equivalents, physical exam, ECG, renal function, and troponin levels. These variables can be used to estimate the risk of death and nonfatal cardiac ischemic events: for example, using the TIMI risk score or the Global Registry of Acute Coronary Events (GRACE) risk model.[2]Rao SV, O'Donoghue ML, Ruel M, et al. 2025 ACC/AHA/ACEP/NAEMSP/SCAI guideline for the management of patients with acute coronary syndromes: a report of the American College of Cardiology/American Heart Association Joint Committee on Clinical Practice Guidelines. Circulation. 2025 Apr;151(13):e771-862.
https://www.ahajournals.org/doi/full/10.1161/CIR.0000000000001309?rfr_dat=cr_pub++0pubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org
http://www.ncbi.nlm.nih.gov/pubmed/40014670?tool=bestpractice.com
[92]Antman EM, Cohen M, Bernink PJ, et al. The TIMI risk score for unstable angina/non-ST elevation MI: a method for prognostication and therapeutic decision making. JAMA. 2000 Aug 16;284(7):835-42.
https://jamanetwork.com/journals/jama/fullarticle/192996
http://www.ncbi.nlm.nih.gov/pubmed/10938172?tool=bestpractice.com
[
Thrombolysis in Myocardial Infarction (TIMI) Score for Unstable Angina Non ST Elevation Myocardial Infarction
Opens in new window
]
[
GRACE Score for Acute Coronary Syndrome Prognosis
Opens in new window
]
The TIMI risk score is composed of 7 risk indicators rated on presentation. One point is awarded for the presence of each of the following criteria:
Age >65 years
Presence of ≥3 CAD risk factors
Prior coronary stenosis >50%
ST-segment deviation on ECG
Elevated serum cardiac biomarkers
At least 2 anginal episodes in the past 24 hours
Use of aspirin in the past 7 days.
Patients with a TIMI score of 0 to 2 are low risk, 3 to 4 are intermediate risk, and 5 to 7 are high risk. All-cause mortality, rate of MI, and rate of urgent revascularization at 14 days increase in proportion to the number of risk factors present on the TIMI score.
The GRACE risk model is a web-based tool that can be used to predict in-hospital and post-discharge mortality or MI in patients following an initial ACS.
The Killip classification is another tool that can be used for risk stratification. This classification system risk stratifies patients with acute MI based on clinical evidence of left ventricular failure:
Class I: no evidence of CHF
Class II: presence of a third heart sound gallop, basilar rales, or elevated jugular venous pressure
Class III: presence of pulmonary edema
Class IV: cardiogenic shock.
The HEART Score incorporates elements of the patient’s history, ECG, age, risk factors, and troponin and is used for patients in the emergency room setting to assess risk of acute MI, percutaneous coronary intervention, coronary artery bypass graft (CABG), and death within 6 weeks of initial presentation.[93]Backus BE, Six AJ, Kelder JC, et al. A prospective validation of the HEART score for chest pain patients at the emergency department. Int J Cardiol. 2013 Oct 3;168(3):2153-8.
https://www.internationaljournalofcardiology.com/article/S0167-5273(13)00315-X/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/23465250?tool=bestpractice.com
[
HEART Score
Opens in new window
]
Do not order a coronary artery calcium test in patients with known atherosclerotic disease, including those with stents and bypass grafts, as this offers limited incremental prognostic value for these individuals.[94]American College of Cardiology. Five things physicians and patients should question. Choosing Wisely, an initiative of the ABIM Foundation. 2023 [internet publication].
https://web.archive.org/web/20230402075927/https://www.choosingwisely.org/societies/american-college-of-cardiology
[95]Society of Cardiovascular Computed Tomography. Five things physicians and patients should question. Choosing Wisely, an initiative of the ABIM Foundation. 2021 [internet publication].
https://web.archive.org/web/20221003082824/https://www.choosingwisely.org/societies/society-of-cardiovascular-computed-tomography
[96]Orringer CE, Blaha MJ, Blankstein R, et al. The National Lipid Association scientific statement on coronary artery calcium scoring to guide preventive strategies for ASCVD risk reduction. J Clin Lipidol. 2021 Jan-Feb;15(1):33-60.
https://www.lipidjournal.com/article/S1933-2874(20)30342-1/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/33419719?tool=bestpractice.com
Subsequent tests
Following initial workup and risk stratification, a range of additional investigations may be considered.
Brain natriuretic peptide (BNP) and N-terminal pro-BNP (NT-pro-BNP): measurement of BNP or NT-pro-BNP may be considered to supplement assessment of global risk in patients with suspected ACS, in particular to predict risk of mortality, acute heart failure and development of atrial fibrillation (AF).[5]Byrne RA, Rossello X, Coughlan JJ, et al. 2023 ESC guidelines for the management of acute coronary syndromes. Eur Heart J. 2023 Oct 12;44(38):3720-826.
https://academic.oup.com/eurheartj/article/44/38/3720/7243210
Lipid profile: this test is indicated in the first 24 hours of admission to the hospital to assess for lipid abnormalities and therefore the need for any lipid-lowering therapy.
Angiography: urgent and immediate angiography is indicated if the patients is clinically unstable or has any very high risk features: ongoing or recurrent pain despite treatment, hemodynamic instability (low blood pressure or shock) or cardiogenic shock, recurrent dynamic ECG changes suggestive of ischemia, a life-threatening arrhythmia (ventricular tachycardia or ventricular fibrillation) or cardiac arrest after presentation, or mechanical complications such as new onset mitral regurgitation.[2]Rao SV, O'Donoghue ML, Ruel M, et al. 2025 ACC/AHA/ACEP/NAEMSP/SCAI guideline for the management of patients with acute coronary syndromes: a report of the American College of Cardiology/American Heart Association Joint Committee on Clinical Practice Guidelines. Circulation. 2025 Apr;151(13):e771-862.
https://www.ahajournals.org/doi/full/10.1161/CIR.0000000000001309?rfr_dat=cr_pub++0pubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org
http://www.ncbi.nlm.nih.gov/pubmed/40014670?tool=bestpractice.com
[5]Byrne RA, Rossello X, Coughlan JJ, et al. 2023 ESC guidelines for the management of acute coronary syndromes. Eur Heart J. 2023 Oct 12;44(38):3720-826.
https://academic.oup.com/eurheartj/article/44/38/3720/7243210
[97]Lawton JS, Tamis-Holland JE, Bangalore S, et al. 2021 ACC/AHA/SCAI guideline for coronary artery revascularization: a report of the American College of Cardiology/American Heart Association Joint Committee On Clinical Practice Guidelines. Circulation. 2022 Jan 18;145(3):e18-114.
https://www.ahajournals.org/doi/full/10.1161/CIR.0000000000001038
http://www.ncbi.nlm.nih.gov/pubmed/34882435?tool=bestpractice.com
Patients with a history of anaphylaxis or allergy to contrast must be premedicated prior to angiography.[Figure caption and citation for the preceding image starts]: 64-slice CT angiography (A) and conventional angiography (B) showing a high-grade lesion in the mid-right coronary artery, indicated by the arrows. The arrowheads show artifacts that may be mistaken for lesionsFrom: Schussler JM and Grayburn PA. Heart. 2007 Mar;93(3):290-7 [Citation ends].
[Figure caption and citation for the preceding image starts]: 64-slice CT angiography of a patient with stable angina showing 3D reconstruction (A), curved reformatted images (B) and confirmation of a high-grade lesion on conventional angiography (C). The arrowheads show calcified plaques. Dx= diagnosisFrom: Schussler JM and Grayburn PA. Heart. 2007 Mar;93(3):290-7 [Citation ends].
Stress testing: stress testing, including treadmill exercise testing, may be useful and is recommended in low and intermediate pretest probability with a normal ECG and normal high sensitivity biomarkers to assist with guiding the need for an invasive strategy.[2]Rao SV, O'Donoghue ML, Ruel M, et al. 2025 ACC/AHA/ACEP/NAEMSP/SCAI guideline for the management of patients with acute coronary syndromes: a report of the American College of Cardiology/American Heart Association Joint Committee on Clinical Practice Guidelines. Circulation. 2025 Apr;151(13):e771-862.
https://www.ahajournals.org/doi/full/10.1161/CIR.0000000000001309?rfr_dat=cr_pub++0pubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org
http://www.ncbi.nlm.nih.gov/pubmed/40014670?tool=bestpractice.com
[98]Lindahl B, Andrén B, Ohlsson J, et al. Risk stratification in unstable coronary artery disease. Additive value of troponin T determinations and pre-discharge exercise tests. Eur Heart J. 1997 May;18(5):762-70.
http://www.ncbi.nlm.nih.gov/pubmed/9152646?tool=bestpractice.com
[99]Nyman I, Larsson H, Areskog M, et al. The predictive value of silent ischemia at an exercise test before discharge after an episode of unstable coronary artery disease. Am Heart J. 1992 Feb;123(2):324-31.
http://www.ncbi.nlm.nih.gov/pubmed/1736566?tool=bestpractice.com
The sensitivity and specificity of these tests increase when combined with either nuclear imaging to look for myocardial perfusion defects or echocardiography to assess wall motion abnormalities. The key positive finding on nuclear imaging stress tests is the presence of a reversible defect. This is an area of myocardium that becomes deprived of perfusion during increased myocardial demand and reperfuses on stopping the activity. This signifies stenosis within the coronary circulation that may be treated with percutaneous coronary intervention or CABG. Submaximal exercise testing can be performed at 4-7 days after myocardial infarction, while symptom limited testing can be performed at 14-21 days post-myocardial infarction, when the patient has been free of active ischemic or heart failure symptoms.[100]Fletcher GF, Ades PA, Kligfield P, et al. Exercise standards for testing and training: a scientific statement from the American Heart Association. Circulation. 2013 Aug 20;128(8):873-934.
https://www.ahajournals.org/doi/full/10.1161/cir.0b013e31829b5b44
http://www.ncbi.nlm.nih.gov/pubmed/23877260?tool=bestpractice.com
High risk patients should be considered for invasive strategy instead of stress testing.[101]Society for Cardiovascular Magnetic Resonance. Five things physicians and patients should question. Choosing Wisely, an initiative of the ABIM Foundation. 2014 [internet publication].
https://web.archive.org/web/20221205222423/https://www.choosingwisely.org/societies/society-for-cardiovascular-magnetic-resonance
Coronary CT angiography (CCTA): this may provide noninvasive evaluation of coronary anatomy and atherosclerosis.[102]Raff GL, Gallagher MJ, O'Neill WW, et al. Diagnostic accuracy of noninvasive coronary angiography using 64-slice spiral computed tomography. J Am Coll Cardiol. 2005 Aug 2;46(3):552-7.
http://www.onlinejacc.org/content/46/3/552
http://www.ncbi.nlm.nih.gov/pubmed/16053973?tool=bestpractice.com
Renal failure is a relative contraindication. Patients with contrast allergy may be premedicated prior to angiography. Due to the high negative predictive value of CCTA, evidence suggests that CCTA is useful in patients with low to moderate risk of NSTEMI. When compared with the standard care of low-risk patients (observation, serial enzymes followed by stress testing) CCTA reduced time to diagnosis, reduced length of emergency department stay, and had similar safety.[103]Hoffmann U, Truong QA, Schoenfeld DA, et al; ROMICAT-II Investigators. Coronary CT angiography versus standard evaluation in acute chest pain. N Engl J Med. 2012 Jul 26;367(4):299-308.
https://www.nejm.org/doi/10.1056/NEJMoa1201161
http://www.ncbi.nlm.nih.gov/pubmed/22830462?tool=bestpractice.com
CCTA is not indicated for patients with high-risk features (i.e., ischemic ECG changes, positive cardiac markers) so should not be used in high-risk emergency department patients presenting with acute chest pain.[5]Byrne RA, Rossello X, Coughlan JJ, et al. 2023 ESC guidelines for the management of acute coronary syndromes. Eur Heart J. 2023 Oct 12;44(38):3720-826.
https://academic.oup.com/eurheartj/article/44/38/3720/7243210
[84]Edvardsen T, Asch FM, Davidson B, et al. Non-invasive imaging in coronary syndromes: recommendations of the European Association of Cardiovascular Imaging and the American Society of Echocardiography, in collaboration with the American Society of Nuclear Cardiology, Society of Cardiovascular Computed Tomography, and Society for Cardiovascular Magnetic Resonance. Eur Heart J Cardiovasc Imaging. 2022 Jan 24;23(2):e6-33.
https://academic.oup.com/ehjcimaging/article/23/2/e6/6423983
http://www.ncbi.nlm.nih.gov/pubmed/34751391?tool=bestpractice.com
[95]Society of Cardiovascular Computed Tomography. Five things physicians and patients should question. Choosing Wisely, an initiative of the ABIM Foundation. 2021 [internet publication].
https://web.archive.org/web/20221003082824/https://www.choosingwisely.org/societies/society-of-cardiovascular-computed-tomography
[104]Hendel RC, Patel MR, Kramer CM, et al; American College of Cardiology Foundation Quality Strategic Directions Committee Appropriateness Criteria Working Group; American College of Radiology; Society of Cardiovascular Computed Tomography; Society for Cardiovascular Magnetic Resonance; American Society of Nuclear Cardiology; North American Society for Cardiac Imaging; Society for Cardiovascular Angiography and Interventions; Society of Interventional Radiology. ACCF/ACR/SCCT/SCMR/ASNC/NASCI/SCAI/SIR 2006 appropriateness criteria for cardiac computed tomography and cardiac magnetic resonance imaging. J Am Coll Cardiol. 2006 Oct 3;48(7):1475-97.
http://www.onlinejacc.org/content/48/7/1475
http://www.ncbi.nlm.nih.gov/pubmed/17010819?tool=bestpractice.com
[Figure caption and citation for the preceding image starts]: 64-slice CT angiography of a patient with stable angina showing 3D reconstruction (A), curved reformatted images (B) and confirmation of a high-grade lesion on conventional angiography (C). The arrowheads show calcified plaques. Dx= diagnosisFrom: Schussler JM and Grayburn PA. Heart. 2007 Mar;93(3):290-7 [Citation ends].
[Figure caption and citation for the preceding image starts]: 64-slice CT angiography (A) and conventional angiography (B) showing a high-grade lesion in the mid-right coronary artery, indicated by the arrows. The arrowheads show artifacts that may be mistaken for lesionsFrom: Schussler JM and Grayburn PA. Heart. 2007 Mar;93(3):290-7 [Citation ends].