Tests
1st tests to order
complete blood count
comprehensive metabolic panel
CT chest/abdomen/pelvis
Test
CT scan of the chest, abdomen, and pelvis, with intravenous contrast, should be performed as part of the initial diagnostic workup.[2]
[Figure caption and citation for the preceding image starts]: CT abdomen with intravenous contrast, revealing numerous enhancing lesions in the right hepatic lobe, with associated ascites. Percutaneous biopsy of one of these hepatic lesions revealed adenocarcinoma, but no primary site was identified during routine workup. This is a typical presentation of ACUPFrom the personal collection of Dr D. Cosgrove [Citation ends].
[Figure caption and citation for the preceding image starts]: CT abdomen with intravenous contrast, revealing numerous enhancing liver lesions in both hepatic lobes. Percutaneous biopsy of a right lobe lesion revealed adenocarcinomaFrom the personal collection of Dr D. Cosgrove [Citation ends].
Result
abnormal masses
MRI
biopsy (pathologic assessment)
Test
Diagnosis of adenocarcinoma of unknown primary site (ACUP) requires an accurate pathologic assessment of a biopsy specimen.[2]
The specimen should be obtained using core needle biopsy (preferred) and/or fine-needle aspiration from the most easily accessible metastatic site.[2] A core needle, incisional, or excisional biopsy may be performed if additional biopsy material is required.
Biopsy samples should be examined initially by light microscopy to confirm the histologic subtype (i.e., adenocarcinoma).[2]
Light microscopy and standard hematoxylin and eosin staining can distinguish tumors with obvious gland formation (well-differentiated to moderately differentiated adenocarcinoma) from those with scant glandular structures, consistent with adenocarcinoma due to mucin production (poorly differentiated or undifferentiated adenocarcinoma).
The degree of differentiation of the glandular structures is determined by the pathologist, and is typically classified as well-, moderately, or poorly differentiated, or undifferentiated.[29]
Result
may show well-, moderately, or poorly differentiated, or undifferentiated adenocarcinoma
immunohistochemistry (IHC) tests
Test
Once a histologic diagnosis of adenocarcinoma has been confirmed, IHC testing for specific biomarkers is performed. Biopsy specimens may provide information about tumor lineage and cell (tumor) type to help identify the likely primary tumor site.[2] In some patients, IHC testing may be performed to identify potentially actionable tumor markers (e.g., RAS, HER2, or ALK rearrangements).
Testing for specific IHC markers should follow a stepwise approach based on findings from the initial diagnostic work-up and in consultation with a pathologist.[2][3]
Testing a large series of IHC markers should be avoided.[2]
The following IHC markers may be used to identify tumor lineage and likely primary site:[2]
Pan-keratin (AE1/AE3 and CAM5.2): can indicate a carcinoma lineage.
Cytokeratin-7 (CK7): expression is generally limited to lung, breast, ovary, endometrium, and upper gastrointestinal/pancreaticobiliary cancers, and is not seen in lower gastrointestinal tract tumors.
Cytokeratin-20 (CK20): generally expressed on gastrointestinal epithelium, urothelium, and Merkel cells.
CDX2: suggests colorectal or other gastrointestinal tract primary, or pancreaticobiliary tract primary.
SATB2: suggests colorectal or other gastrointestinal tract primary, or osteosarcoma primary.
TTF-1: suggestive of lung or thyroid primary.
Napsin A: suggests lung primary.
GATA3: suggests breast, urinary bladder, or salivary gland primary.
PAX8: suggests renal, ovary, endometrial, cervix, thymus, or thyroid primary.
Estrogen receptor/progesterone receptor: suggests breast, ovary, or endometrial primary.
A more extensive list of IHC markers is available in published guidelines.[2][3][23]
Information garnered from these tests must be considered in conjunction with the clinical scenario and other test results.
If initial IHC markers narrow the differential of likely primary sites, more specific antibodies can be utilized to further solidify the diagnosis.[24] Results of further testing may not alter treatment, therefore caution must be taken to avoid unnecessary delay to treatment initiation.
IHC marker profiles may occasionally differ between primary and metastatic lesions.[25][26][27]
Result
specific tumor antigens are identified via labeled antibodies
Tests to consider
fecal occult blood test
Test
Ordered if a colorectal primary is suspected.[2]
Result
may be positive
lactate dehydrogenase
urinalysis
Test
Can be used to assess renal function, detect paraneoplastic syndromes, or evaluate for associated metabolic abnormalities.[2]
Result
normal or abnormal
18F-fluorodeoxyglucose-positron emission tomography (FDG-PET)/CT
breast imaging (mammography, MRI, ultrasound)
Test
Mammography is required for patients with a clinical presentation suggestive of breast cancer (e.g., axillary, mediastinal, and/or supraclavicular nodal involvement).[2][3][21]
Contrast-enhanced breast MRI and/or breast ultrasound is indicated if mammography is not diagnostic but there is histopathologic evidence for breast cancer.[2]
Result
may show microcalcifications (on mammography) or a mass lesion, which suggests breast cancer
transvaginal ultrasound
Test
Transvaginal ultrasound is the preferred imaging modality for women presenting with ascites to assess for the presence of an ovarian mass.
Color Doppler should be included in the examination.[22]
Result
may show an adnexal mass, which suggests ovarian cancer
paracentesis
Test
Recommended (if technically possible) in all patients presenting with ascites to assess for peritoneal adenocarcinoma.[21]
Larger volume (>1 liter) paracentesis increases the diagnostic yield.
Result
presence of adenocarcinoma cells in the peritoneal fluid
endoscopy
Test
Endoscopy should be considered if immunohistochemistry tests suggest an upper gastrointestinal or colorectal primary, or if patients have certain clinical findings (e.g., liver metastases; positive supraclavicular nodes).[2]
Result
may show lesions for gastrointestinal or colorectal primary
direct laryngoscopy with or without esophagoscopy and bronchoscopy
Test
Recommended for patients with cervical lymphadenopathy.[19]
The extent of examinations is dependent on the level of cervical nodes involved.
Additional immunohistochemical testing can help to confirm an occult head and neck primary tumor.
Identified lesions should be biopsied to confirm pathologic concordance with the metastatic focus.
Result
may show mucosal lesions for primary head and neck carcinoma, esophageal carcinoma, or lung carcinoma
serum tumor markers
Test
Assessment of serum tumor markers may be considered in specific clinical scenarios to identify a possible primary site.[2] However, they are nonspecific and have no diagnostic role in patients with adenocarcinoma of unknown primary (ACUP).
Prostate-specific antigen (PSA) should be measured to assess for possible prostate primary in the following patients: men age >40 years with ACUP (except for those with metastases limited to the liver or brain); and all men with ACUP presenting with bone metastases or multiple sites of involvement.[2]
Serum alpha-fetoprotein (AFP) and beta-human chorionic gonadotropin (beta-hCG) should be measured (followed by testicular ultrasound, if AFP or beta-hCG is elevated) to assess for possible testicular germ cell primary in the following patients: men age <50 years with ACUP presenting with positive mediastinal nodes; and men age <65 years with ACUP presenting with retroperitoneal mass.[2]
CA-125 should be measured to assess for possible ovarian primary (in consultation with a gynecologic oncologist) in the following patients with ovaries: women age <50 years with ACUP presenting with positive mediastinal nodes; women of any age with ACUP presenting with metastases in the inguinal nodes, chest (multiple nodules), or peritoneum (with or without ascites); and women with ACUP presenting with pleural effusion or retroperitoneal mass.[2]
Testing for carcinoembryonic antigen (CEA), CA 19-9, and CA 15-3 has no diagnostic role but may be useful in certain circumstances; for example, in assessing treatment response, whereby consideration may be given to establishing a baseline during diagnostic workup.[2]
Result
may show elevated serum tumor markers (e.g., PSA, AFP, beta-hCG, CA-125)
genetic biomarker testing
Test
Can be performed to identify genetic biomarkers to guide use of biomarker-driven targeted therapy and immunotherapy.[2]
Microsatellite instability (MSI)/mismatch repair (MMR) testing can guide use of immune checkpoint inhibitors.
Tumor mutational burden (TMB) analysis (using validated and/or Food and Drug Administration [FDA] approved assays) can guide use of immune checkpoint inhibitors.
Molecular profiling (e.g., using next-generation sequencing [NGS] assays) can identify actionable genetic mutations (e.g., BRAF V600E) and alterations (e.g., NTRK or RET gene fusions). Considered after histology is confirmed.
Result
may show genetic biomarkers associated with response to targeted therapy and immunotherapy (e.g., BRAF V600E mutations; NTRK gene fusions; RET gene fusions; TMB-high [≥10 mutations/megabase]; MSI-high/MMR-deficient)
Emerging tests
tissue of origin testing
Test
Tissue of origin testing (e.g., using gene expression profiling and machine learning) has been investigated in patients with cancer of unknown primary to predict a likely primary site and guide site-specific therapy.[30][31][32] Although diagnostic benefit has been demonstrated with tissue of origin testing, clinical benefit has been mixed.
Tissue of origin testing is currently not recommended in patients with adenocarcinoma of unknown primary site.[2][3]
Result
may show molecular profile for a likely primary site
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