Diagnosis of genital warts is made based on the clinical presentation of lesions located on the genital area, perianal region, or adjacent areas such as the mons pubis, with a tendency for genital wart formation to occur on areas of high friction.[3]Wiley DJ, Douglas J, Beutner K, et al. External genital warts: diagnosis, treatment, and prevention. Clin Infect Dis. 2002 Oct 15;35(Suppl 2):S210-24.
https://academic.oup.com/cid/article/35/Supplement_2/S210/316436
http://www.ncbi.nlm.nih.gov/pubmed/12353208?tool=bestpractice.com
[17]Ahmed AM, Madkan V, Tyring SK. Human papillomaviruses and genital disease. Dermatol Clin. 2006 Apr;24(2):157-65.
http://www.ncbi.nlm.nih.gov/pubmed/16677964?tool=bestpractice.com
[38]Brodell LA, Mercurio MG, Brodell RT. The diagnosis and treatment of human papillomavirus-mediated genital lesions. Cutis. 2007 Apr;79(4 Suppl):5-10.
http://www.ncbi.nlm.nih.gov/pubmed/17508490?tool=bestpractice.com
[39]Steinberg JL, Cibley LJ, Rice PA. Genital warts: diagnosis, treatment, and counseling for the patient. Curr Clin Top Infect Dis. 1993;13:99-122.
http://www.ncbi.nlm.nih.gov/pubmed/7691082?tool=bestpractice.com
Biopsy is generally not performed for the diagnosis of genital warts.
Clinical presentation
Genital warts are usually 1 to 3 mm, discrete, sessile, smooth-surfaced exophytic papillomas or they may coalesce into larger plaques. These plaques may be extensive, with expansion into the urethra, or into the anal or vaginal canals.[3]Wiley DJ, Douglas J, Beutner K, et al. External genital warts: diagnosis, treatment, and prevention. Clin Infect Dis. 2002 Oct 15;35(Suppl 2):S210-24.
https://academic.oup.com/cid/article/35/Supplement_2/S210/316436
http://www.ncbi.nlm.nih.gov/pubmed/12353208?tool=bestpractice.com
[Figure caption and citation for the preceding image starts]: Venereal warts in the anal region of the perineumCDC/Dr Wiesner [Citation ends].
Color varies from flesh-colored to whitish to hyperpigmented. Maceration may occur, particularly in moist areas lacking the thick horny cell layer found in cutaneous warts.[5]Kirnbauer R, Lenz P, Okun MM. Human papillomavirus. In: Bolognia JL, Jorizzo JL, Rapini RP, eds. Dermatology Vol 1. London: Mosby; 2003:1217-33.[Figure caption and citation for the preceding image starts]: Wart on shaft of penisFrom Dr Tyring's personal collection; used with permission [Citation ends].
[Figure caption and citation for the preceding image starts]: Close-up of penile wartFrom Dr Tyring's personal collection; used with permission [Citation ends].
External genital warts may be diagnosed using direct visual inspection aided by bright light and magnification.[3]Wiley DJ, Douglas J, Beutner K, et al. External genital warts: diagnosis, treatment, and prevention. Clin Infect Dis. 2002 Oct 15;35(Suppl 2):S210-24.
https://academic.oup.com/cid/article/35/Supplement_2/S210/316436
http://www.ncbi.nlm.nih.gov/pubmed/12353208?tool=bestpractice.com
Lesions are generally asymptomatic, but may be painful, friable, or pruritic.[5]Kirnbauer R, Lenz P, Okun MM. Human papillomavirus. In: Bolognia JL, Jorizzo JL, Rapini RP, eds. Dermatology Vol 1. London: Mosby; 2003:1217-33.[6]Beutner KR, Reitano MV, Richwald GA, et al. External genital warts: report of the American Medical Association consensus conference. Clin Infect Dis. 1998 Oct;27(4):796-806.
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Bleeding may occur due to local trauma or maceration of the area. Urinary symptoms such as terminal hematuria or abnormal stream of urine may be present.
Investigations
A biopsy may be indicated if the genital warts appear fixed to underlying structures or are refractory to standard therapy.[40]Beutner KR, Wiley DJ, Douglas JM, et al. Genital warts and their treatment. Clin Infect Dis. 1999 Jan;28 Suppl 1:S37-56.
http://www.ncbi.nlm.nih.gov/pubmed/10028109?tool=bestpractice.com
Additional indications for biopsy include ulceration of the lesions or an individual wart larger than 1 cm.[6]Beutner KR, Reitano MV, Richwald GA, et al. External genital warts: report of the American Medical Association consensus conference. Clin Infect Dis. 1998 Oct;27(4):796-806.
http://www.ncbi.nlm.nih.gov/pubmed/9798036?tool=bestpractice.com
Should a biopsy be indicated, the following histologic features are seen: epidermal hyperplasia, parakeratosis, koilocytosis, and papillomatosis.[17]Ahmed AM, Madkan V, Tyring SK. Human papillomaviruses and genital disease. Dermatol Clin. 2006 Apr;24(2):157-65.
http://www.ncbi.nlm.nih.gov/pubmed/16677964?tool=bestpractice.com
Compared with common warts, the papillomatosis seen in genital warts tends to be more rounded. Not all of these histologic features are necessarily seen in every genital wart. Should the biopsy be definitive for condyloma, do not perform immunohistochemistry (IHC) or in situ hybridization (ISH) for HPV.[41]American Society of Dermatopathology. Choosing Wisely, an initiative of the ABIM Foundation. 2023 [internet publication].
https://web.archive.org/web/20230408003146/https://www.choosingwisely.org/clinician-lists/8-1-do-not-routinely-perform-immunohistochemistry-ihc-or-in-situ-hybridization-ish-for-human-papilloma-virus-hpv-solely-as-a-diagnostic-adjunct-or-ancillary-test-on-the-lesion-from-a
[42]Vidal CI, Armbrect EA, Andea AA, et al. Appropriate use criteria in dermatopathology: Initial recommendations from the American Society of Dermatopathology. J Cutan Pathol. 2018 Aug;45(8):563-80.
https://deepblue.lib.umich.edu/handle/2027.42/145218
http://www.ncbi.nlm.nih.gov/pubmed/29566273?tool=bestpractice.com
Do not perform low-risk HPV testing (testing for strains of HPV that cause genital warts or minor cell changes on the cervix) because there is no change in management indicated when low-risk HPV is identified.[43]American Society for Clinical Pathology. Thirty five things physicians and patients should question. Choosing Wisely, an initiative of the ABIM Foundation. 2021 [internet publication].
https://web.archive.org/web/20230316185857/https://www.choosingwisely.org/societies/american-society-for-clinical-pathology
[44]Workowski KA, Bachmann LH, Chan PA, et al. Sexually transmitted infections treatment guidelines, 2021. MMWR Recomm Rep. 2021 Jul 23;70(4):1-187.
ttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8344968
http://www.ncbi.nlm.nih.gov/pubmed/34292926?tool=bestpractice.com
In patients with recurrent perianal warts, evaluation for intra-anal warts by anoscopy is recommended.[6]Beutner KR, Reitano MV, Richwald GA, et al. External genital warts: report of the American Medical Association consensus conference. Clin Infect Dis. 1998 Oct;27(4):796-806.
http://www.ncbi.nlm.nih.gov/pubmed/9798036?tool=bestpractice.com
Urinary symptoms such as terminal hematuria or abnormal stream of urine should prompt a referral for urethroscopy to evaluate the distal urethra and meatus.[6]Beutner KR, Reitano MV, Richwald GA, et al. External genital warts: report of the American Medical Association consensus conference. Clin Infect Dis. 1998 Oct;27(4):796-806.
http://www.ncbi.nlm.nih.gov/pubmed/9798036?tool=bestpractice.com