There is debate over the exact number of bacteria in a urine culture that is needed to define urinary tract infection (UTI) in men. The standardised threshold in symptomatic patients is >10⁵ colony-forming units/mL (CFU/mL) for organisms identified as common pathogens; however, many sites now use either 10⁴ CFU/mL or 10³ CFU/mL threshold, based on the method of collection or patient population as a baseline for culture work-up and clinical significance.[51]Miller JM, Binnicker MJ, Campbell S, et al. Guide to utilization of the microbiology laboratory for diagnosis of infectious diseases: 2024 update by the Infectious Diseases Society of America (IDSA) and the American Society for Microbiology (ASM). Clin Infect Dis. 2024 Mar 5:ciae104.
https://academic.oup.com/cid/advance-article/doi/10.1093/cid/ciae104/7619499
http://www.ncbi.nlm.nih.gov/pubmed/38442248?tool=bestpractice.com
In men, positive results are usually seen if there are more than 10³ CFU/mL, which is much lower than the threshold for women.[52]Johnson JR. Laboratory diagnosis of urinary tract infections in adult patients. Clin Infect Dis. 2004 Sep 15;39(6):873; author reply 873-4.
A positive diagnosis of UTI in a male can be made in a symptomatic patient with urine that grows ≥10³ CFU/mL of one, or predominantly one, organism in culture.[31]Lipsky BA, Schaberg DR. Managing urinary tract infections in men. Hosp Prac. 2000 Jan 15;35(1):53-9.
http://www.ncbi.nlm.nih.gov/pubmed/10645989?tool=bestpractice.com
[52]Johnson JR. Laboratory diagnosis of urinary tract infections in adult patients. Clin Infect Dis. 2004 Sep 15;39(6):873; author reply 873-4.[53]Orenstein R, Wong ES. Urinary tract infections in adults. Am Fam Physician. 1999 Mar 1;59(5):1225-34.
https://www.aafp.org/pubs/afp/issues/1999/0301/p1225.html
http://www.ncbi.nlm.nih.gov/pubmed/10088877?tool=bestpractice.com
History
Dysuria most often results from localised infection.[2]European Association of Urology. Guidelines on urological infections. Mar 2025 [internet publication].
https://uroweb.org/guidelines/urological-infections
[8]Roberts RG, Hartlaub PP. Evaluation of dysuria in men. Am Fam Physician. 1999 Sep 1;60(3):865-72.
http://www.ncbi.nlm.nih.gov/pubmed/10498112?tool=bestpractice.com
Also, frequency, urgency, and suprapubic pain signal UTI. Costovertebral angle pain suggests extension of UTI to the kidney (pyelonephritis). Rectal or perineal pain can indicate UTI associated with prostatitis. Men may present with urethral discharge or have symptoms related to impaired urine flow, such as hesitancy or nocturia.[31]Lipsky BA, Schaberg DR. Managing urinary tract infections in men. Hosp Prac. 2000 Jan 15;35(1):53-9.
http://www.ncbi.nlm.nih.gov/pubmed/10645989?tool=bestpractice.com
A history of cloudy or foul smelling urine can indicate a UTI.[9]American Urological Association. Urinary tract infections in adults. Nov 2022 [internet publication].
https://www.urologyhealth.org/urology-a-z/u/urinary-tract-infections-in-adults
Finally, the history includes identification of systemic signs (e.g., fever, rigors), presence of haematuria, and possible immunocompromised states (e.g., diabetes mellitus) that may indicate a more severely ill patient requiring hospitalisation.
The past medical history can reveal the following risks contributing to UTI:[31]Lipsky BA, Schaberg DR. Managing urinary tract infections in men. Hosp Prac. 2000 Jan 15;35(1):53-9.
http://www.ncbi.nlm.nih.gov/pubmed/10645989?tool=bestpractice.com
The social history will identify sexual practices and preferences; anal sex in particular increases risk of UTI.
Physical examination
The physical examination is useful in excluding other possible causes for the patient's symptoms. It should at least include the abdomen, genitalia, rectum, and palpation of the costovertebral angle.
A tender boggy prostate, firm enlarged prostate, or nodularity suggests prostatitis, prostate hyperplasia, and prostate cancer, respectively.
Penile lesions or discharge suggest sexually transmitted infection.
Tenderness or swelling of the epididymis or testes implies the presence of epididymitis or orchitis, respectively.
Fever is suggestive of a systemic bacterial infection, and may be seen with prostatitis, orchitis, epididymitis, pyelonephritis, or UTI.
Laboratory
A dipstick or microscopic urinalysis (U/A) is the initial test for men with suspected UTI. If dipstick is negative for nitrites and leukocyte esterase, or microscopic U/A is negative for bacteria and leukocytes, this excludes infection, but the presence of these markers does not rule in UTI.[4]Hummers-Pradier E, Kochen MM. Urinary tract infections in adult general practice patients. Br J Gen Pract. 2002 Sep;52(482):752-61.
http://bjgp.org/content/bjgp/52/482/752.full.pdf
http://www.ncbi.nlm.nih.gov/pubmed/12236281?tool=bestpractice.com
[54]Devillé WL, Yzermans JC, van Duijn NP, et al. The urine dipstick test useful to rule out infections: a meta-analysis of the accuracy. BMC Urol. 2004 Jun 2;4:4.
https://bmcurol.biomedcentral.com/articles/10.1186/1471-2490-4-4
http://www.ncbi.nlm.nih.gov/pubmed/15175113?tool=bestpractice.com
One Denmark study of patients presenting to the accident and emergency department found both a negative leukocyte esterase and negative nitrite had a negative predictive value of 93.3% for men.[55]Chernaya A, Søborg C, Midttun M. Validity of the urinary dipstick test in the diagnosis of urinary tract infections in adults. Dan Med J. 2021 Dec 15;69(1):A07210607.
https://ugeskriftet.dk/dmj/validity-urinary-dipstick-test-diagnosis-urinary-tract-infections-adults
http://www.ncbi.nlm.nih.gov/pubmed/34913433?tool=bestpractice.com
Negative results should prompt a search for another cause of the patient's symptoms.
A positive U/A in a man with typical UTI symptoms should be followed by a urine culture and empirical antibiotic therapy while awaiting the culture result. In the absence of signs and symptoms of a UTI, a urine culture is typically not recommended.[51]Miller JM, Binnicker MJ, Campbell S, et al. Guide to utilization of the microbiology laboratory for diagnosis of infectious diseases: 2024 update by the Infectious Diseases Society of America (IDSA) and the American Society for Microbiology (ASM). Clin Infect Dis. 2024 Mar 5:ciae104.
https://academic.oup.com/cid/advance-article/doi/10.1093/cid/ciae104/7619499
http://www.ncbi.nlm.nih.gov/pubmed/38442248?tool=bestpractice.com
In men with a positive U/A, obtaining a Gram stain of the urine can guide the initial antibiotic choice; however, it is not required, because empirical therapy can be chosen based on the anticipated pathogenic bacteria. Gram stain, like U/A, does not confirm the presence of UTI.[23]Cornia PB, Takahashi TA, Lipsky BA. The microbiology of bacteriuria in men: a 5-year study at a Veterans' Affairs hospital. Diagn Microbiol Infect Dis. 2006 Sep;56(1):25-30.
http://www.ncbi.nlm.nih.gov/pubmed/16713165?tool=bestpractice.com
Culture is essential to confirm the diagnosis of UTI and because of the potential for non-traditional organisms in men.[7]Schaeffer AJ. Infections of the urinary tract. In: Walsh PC, ed. Campbells' urology, 8th ed. Philadelphia, PA: Saunders; 2002.[19]Stamm WE, Hooton TM. Management of urinary tract infections in adults. N Engl J Med. 1993 Oct 28;329(18):1328-34.
http://www.ncbi.nlm.nih.gov/pubmed/8413414?tool=bestpractice.com
The presence of ≥10³ CFU/mL of one, or predominantly one, organism in culture confirms UTI in symptomatic men.[56]Schaeffer AJ, Nicolle LE. Urinary tract infections in older men. N Engl J Med. 2016 Jun 2;374(22):2192. A midstream clean-catch urine sample used for culture compares favourably with suprapubic aspiration or catheter specimens.[57]Lipsky BA, Ireton RC, Fihn SD, et al. Diagnosis of bacteriuria in men: specimen collection and culture interpretation. J Infect Dis. 1987 May;155(5):847-54.
http://www.ncbi.nlm.nih.gov/pubmed/3559288?tool=bestpractice.com
In the absence of signs and symptoms of a UTI, a urine culture is typically not recommended.[51]Miller JM, Binnicker MJ, Campbell S, et al. Guide to utilization of the microbiology laboratory for diagnosis of infectious diseases: 2024 update by the Infectious Diseases Society of America (IDSA) and the American Society for Microbiology (ASM). Clin Infect Dis. 2024 Mar 5:ciae104.
https://academic.oup.com/cid/advance-article/doi/10.1093/cid/ciae104/7619499
http://www.ncbi.nlm.nih.gov/pubmed/38442248?tool=bestpractice.com
The general approach to patients is similar in the outpatient and long-term care settings. However, in long-term care, U/A is even less predictive of the presence of UTI because a high proportion of these patients have pyuria related to asymptomatic bacteriuria.[6]Nicolle LE, Gupta K, Bradley SF, et al. Clinical practice guideline for the management of asymptomatic bacteriuria: 2019 update by the Infectious Diseases Society of America. Clin Infect Dis. 2019 May 2;68(10):e83-110.
https://academic.oup.com/cid/article/68/10/e83/5407612
http://www.ncbi.nlm.nih.gov/pubmed/30895288?tool=bestpractice.com
[16]Shortliffe LM, McCue JD. Urinary tract infection at the age extremes: pediatrics and geriatrics. Am J Med. 2002 Jul 8;113(suppl 1A):55S-66S.
http://www.ncbi.nlm.nih.gov/pubmed/12113872?tool=bestpractice.com
However, a negative U/A does exclude the presence of UTI.[6]Nicolle LE, Gupta K, Bradley SF, et al. Clinical practice guideline for the management of asymptomatic bacteriuria: 2019 update by the Infectious Diseases Society of America. Clin Infect Dis. 2019 May 2;68(10):e83-110.
https://academic.oup.com/cid/article/68/10/e83/5407612
http://www.ncbi.nlm.nih.gov/pubmed/30895288?tool=bestpractice.com
[16]Shortliffe LM, McCue JD. Urinary tract infection at the age extremes: pediatrics and geriatrics. Am J Med. 2002 Jul 8;113(suppl 1A):55S-66S.
http://www.ncbi.nlm.nih.gov/pubmed/12113872?tool=bestpractice.com
In patients with indwelling catheters, a urine dipstick should not be used to diagnose UTI, as catheters are usually colonised with bacteria and will likely give a positive result. Instead, a urine culture is used to support the diagnosis in a symptomatic patient.[58]National Institute for Health and Care Excellence. Urinary tract infections in adults. Feb 2023 [internet publication].
https://www.nice.org.uk/guidance/qs90
Visible or non-visible haematuria need further assessment for malignancy. See Assessment of visible haematuria and Assessment of non-visible haematuria.
Imaging
Imaging of the kidneys, ureters, and bladder by computed tomography (CT), or ultrasound should be reserved for:[5]Ulleryd P, Zackrisson B, Aus G, et al. Selective urological evaluation in men with febrile urinary tract infection. BJU Int. 2001 Jul;88(1):15-20.
http://www.ncbi.nlm.nih.gov/pubmed/11446838?tool=bestpractice.com
[59]Al Lawati H, Blair BM, Larnard J. Urinary tract infections: core curriculum 2024. Am J Kidney Dis. 2024 Jan;83(1):90-100.
https://www.ajkd.org/article/S0272-6386(23)00837-5/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/37906240?tool=bestpractice.com
[60]Expert Panel on Urological Imaging, Alexander LF, Oto A, et al. ACR appropriateness criteria® lower urinary tract symptoms-suspicion of benign prostatic hyperplasia. J Am Coll Radiol. 2019 Nov;16(11s):S378-83.
https://www.jacr.org/article/S1546-1440(19)30621-0/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/31685105?tool=bestpractice.com
[61]Expert Panel on Urological Imaging, Gupta RT, Kalisz K, et al. ACR appropriateness criteria® acute onset flank pain-suspicion of stone disease (urolithiasis). J Am Coll Radiol. 2023 Nov;20(11s):S315-28.
https://www.jacr.org/article/S1546-1440(23)00619-1/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/38040458?tool=bestpractice.com
Those who have voiding dysfunction without a clearly identifiable cause such as BPH
Cases of treatment failure
Suspicion of obstruction or complication
Those with signs of upper tract infection.
Although imaging of men with UTI frequently results in abnormal findings, it usually does not alter treatment. Therefore, it is not indicated in all cases.[32]Andrews SJ, Brooks PT, Hanbury DC, et al. Ultrasonography and abdominal radiography versus intravenous urography in investigation of urinary tract infection in men: prospective incident cohort study. BMJ. 2002 Feb 23;324(7335):454-6.
http://www.bmj.com/content/324/7335/454.full
http://www.ncbi.nlm.nih.gov/pubmed/11859046?tool=bestpractice.com
[59]Al Lawati H, Blair BM, Larnard J. Urinary tract infections: core curriculum 2024. Am J Kidney Dis. 2024 Jan;83(1):90-100.
https://www.ajkd.org/article/S0272-6386(23)00837-5/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/37906240?tool=bestpractice.com
[60]Expert Panel on Urological Imaging, Alexander LF, Oto A, et al. ACR appropriateness criteria® lower urinary tract symptoms-suspicion of benign prostatic hyperplasia. J Am Coll Radiol. 2019 Nov;16(11s):S378-83.
https://www.jacr.org/article/S1546-1440(19)30621-0/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/31685105?tool=bestpractice.com
The American College of Radiology recommends that CT urography and/or ultrasound of the kidneys and retroperitoneum be considered for further evaluation in patients with raised creatinine, stones, haematuria, UTI, or other complicating history.[62]American College of Radiology. ACR appropriateness criteria: lower urinary tract symptoms-suspicion of benign prostatic hyperplasia. 2019 [internet publication].
https://acsearch.acr.org/docs/69368/Narrative
[63]American College of Radiology. ACR appropriateness criteria: acute onset flank pain-suspicion of stone disease (urolithiasis). 2023 [internet publication].
https://acsearch.acr.org/docs/69362/narrative
The healthcare provider must choose an imaging technique based on availability of local resources and the underlying pathology that is suspected.